Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system.

ABSTRACT: Achieving uniform optical resolution for a large tissue sample is a major challenge for deep imaging. For conventional tissue clearing methods, loss of resolution and quality in deep regions is inevitable due to limited transparency. Here we describe the Transparent Embedding Solvent System (TESOS) method, which combines tissue clearing, transparent embedding, sectioning and block-face imaging. We used TESOS to acquire volumetric images of uniform resolution for an adult mouse whole-body sample. The TESOS method is highly versatile and can be combined with different microscopy systems to achieve uniformly high resolution. With a light sheet microscope, we imaged the whole body of an adult mouse, including skin, at a uniform 0.8 × 0.8 × 3.5 μm³ voxel resolution within 120 h. With

SUBMITTER: Yi Y 

PROVIDER: S-EPMC10837210 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature