Ablative fractional laser treatment reduces hedgehog pathway gene expression in murine basal cell carcinomas.

ABSTRACT: This study aimed to investigate the impact of ablative fractional laser (AFL) on hedgehog pathway gene expression in murine microscopic basal cell carcinomas (BCCs) and compare these results to the effect of topical treatment with vismodegib, an FDA-approved hedgehog inhibitor. In 25 mice, 1 cm² skin test sites (n = 44) containing microscopic BCCs were exposed to one of three interventions: a single CO₂ AFL treatment (1 pulse, 40 mJ/microbeam, wavelength 10.6 μm, 5% density, pulse rate 250 Hz, n = 12), eight topical vismodegib treatments (3.8 mg/mL, n = 8), or combination of AFL and vismodegib treatments (n = 9). Untreated controls were included for comparison (n = 15). After 4 days, skin samples were analyzed for hedgehog gene expression (Gli1, Gli2, and Ptch1) by qP

SUBMITTER: Pedersen KK 

PROVIDER: S-EPMC10837214 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature