Mechanism and Effect of HNF4α Decrease in a Rat Model of Cirrhosis and Liver Failure.

ABSTRACT:

Background & aims

HNF4α, a master regulator of liver development and the mature hepatocyte phenotype, is down-regulated in chronic and inflammatory liver disease. We used contemporary transcriptomics and epigenomics to study the cause and effects of this down-regulation and characterized a multicellular etiology.

Methods

Progressive changes in the rat carbon tetrachloride model were studied by deep RNA sequencing and genome-wide chromatin immunoprecipitation sequencing analysis of transcription factor (TF) binding and chromatin modification. Studies compared decompensated cirrhosis with liver failure after 26 weeks of treatment with earlier compensated cirrhosis and with additional rat models of chronic fibrosis. Finally, to resolve cell-specific responses and intercellular

SUBMITTER: Melis M 

PROVIDER: S-EPMC10837635 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature