Single-cell transcriptome analyses reveal critical regulators of spermatogonial stem cell fate transitions.

ABSTRACT:

Background

Spermatogonial stem cells (SSCs) are the foundation cells for continual spermatogenesis and germline regeneration in mammals. SSC activities reside in the undifferentiated spermatogonial population, and currently, the molecular identities of SSCs and their committed progenitors remain unclear.

Results

We performed single-cell transcriptome analysis on isolated undifferentiated spermatogonia from mice to decipher the molecular signatures of SSC fate transitions. Through comprehensive analysis, we delineated the developmental trajectory and identified candidate transcription factors (TFs) involved in the fate transitions of SSCs and their progenitors in distinct states. Specifically, we characterized the A_single spermatogonial subtype marked by the expres

SUBMITTER: Li S 

PROVIDER: S-EPMC10837949 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature