Changes in vocal fold gene expression and histology after injection augmentation in a recurrent laryngeal nerve injury model.

ABSTRACT:

Objective

To investigate changes in neuroregenerative pathways with vocal fold denervation in response to vocal fold augmentation.

Methods

Eighteen Yorkshire crossbreed swine underwent left recurrent laryngeal nerve transection, followed by observation or augmentation with carboxymethylcellulose or calcium hydroxyapatite at two weeks. Polymerase chain reaction expression of genes regulating muscle growth (MyoD1, MyoG and FoxO1) and atrophy (FBXO32) were analysed at 4 and 12 weeks post-injection. Thyroarytenoid neuromuscular junction density was quantified using immunohistochemistry.

Results

Denervated vocal folds demonstrated reduced expression of MyoD1, MyoG, FoxO1 and FBXO32, but overexpression after augmentation. Healthy vocal folds showed increased early and late

SUBMITTER: Malka R 

PROVIDER: S-EPMC10838396 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature