Exploration of neuron heterogeneity in human heart failure with dilated cardiomyopathy through single-cell RNA sequencing analysis.

ABSTRACT:

Objective

We aimed to explore the heterogeneity of neurons in heart failure with dilated cardiomyopathy (DCM).

Methods

Single-cell RNA sequencing (scRNA-seq) data of patients with DCM and chronic heart failure and healthy samples from GSE183852 dataset were downloaded from NCBI Gene Expression Omnibus, in which neuron data were extracted for investigation. Cell clustering analysis, differential expression analysis, trajectory analysis, and cell communication analysis were performed, and highly expressed genes in neurons from patients were used to construct a protein-protein interaction (PPI) network and validated by GSE120895 dataset.

Results

Neurons were divided into six subclusters involved in various biological processes and each subcluster owned its specific cell

SUBMITTER: Cui YH 

PROVIDER: S-EPMC10838417 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature