Identification of the mechanism for dehalorespiration of monofluoroacetate in the phylum Synergistota.

ABSTRACT:

Objective

Monofluoroacetate (MFA) is a potent toxin that blocks ATP production via the Krebs cycle and causes acute toxicity in ruminants consuming MFA-containing plants. The rumen bacterium, Cloacibacillus porcorum strain MFA1 belongs to the phylum Synergistota and can produce fluoride and acetate from MFA as the end-products of dehalorespiration. The aim of this study was to identify the genomic basis for the metabolism of MFA by this bacterium.

Methods

A draft genome sequence for C. porcorum strain MFA1 was assembled and quantitative transcriptomic analysis was performed thus highlighting a candidate operon encoding four proteins that are responsible for the carbon-fluorine bond cleavage. Comparative genome analysis of this operon was undertaken with three other species o

SUBMITTER: Leong LEX 

PROVIDER: S-EPMC10838667 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature