The deubiquitinase OTUB2 promotes cervical cancer growth through stabilizing FOXM1.

ABSTRACT:

Objectives

Ovarian tumor (OTU) domain-containing ubiquitin aldehyde-binding protein Otubain2 (OTUB2) is an important cysteine protease with deubiquitinase activity in the OTU family. However, the role of OTUB2 in cervical cancer (CC) has not been investigated.

Methods

OTUB2 expression was analyzed employing the CC data from The Cancer Genome Atlas (TCGA) database. Western blot and qRT-PCR analysis were performed to identify OTUB2 expression in CC. The oncogenic function of OTUB2 was identified through a series of in vitro and in vivo experiments. Tandem Mass Tag™ Quantitative Proteomics examination was used to identify potential targets of OTUB2.

Results

OTUB2 was overexpressed in CC and was related to poor prognosis of patients. In our in-house cohort,

SUBMITTER: Xiao J 

PROVIDER: S-EPMC10839374 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature