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Protocol for detection of in vitro R-loop formation using dot blots.

ABSTRACT: Dot-blot analysis is a technique that allows for fast and convenient detection and identification of nucleic acids and proteins. Here, we provide a guide for nucleic acid isolation from eukaryotic cells and sample processing to detect RNA/DNA hybrids. We then provide detailed steps to quantify dot signal intensity. This protocol can be adapted for screening conditions that result in the accumulation of R-loops. For complete details on the use and execution of this protocol, please refer to Smith et al.¹.

SUBMITTER: Dowling JW

PROVIDER: S-EPMC10839531 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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Protocol for detection of in vitro R-loop formation using dot blots.

Dowling Jack W JW Smith Julian R JR Forero Adriana A

STAR protocols 20240128 1

Dot-blot analysis is a technique that allows for fast and convenient detection and identification of nucleic acids and proteins. Here, we provide a guide for nucleic acid isolation from eukaryotic cells and sample processing to detect RNA/DNA hybrids. We then provide detailed steps to quantify dot signal intensity. This protocol can be adapted for screening conditions that result in the accumulation of R-loops. For complete details on the use and execution of this protocol, please refer to Smith ...[more]

PMID: 38285737

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