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Nuclear pore complex proteins are involved in centromere distribution.

ABSTRACT: The subnuclear distribution of centromeres is cooperatively regulated by condensin II and the linker of nucleoskeleton and cytoskeleton (LINC) complex. However, other nuclear membrane structures and nuclear proteins are probably involved in centromere dynamics and distribution. Here, we focused on the nuclear pore complex (NPC), which is known to regulate gene expression, transcription memory, and chromatin structure in addition to transport between the cytoplasm and nucleoplasm. We report here that some nucleoporins (Nups), including Nup85, Nup133, CG1, Nup93b, and NUA, are involved in centromere scattering in Arabidopsis thaliana. In addition, the centromere dynamics after metaphase in nup mutants were found to be similar to that of the condensin II mutant. Furthermore, both biochemical and genetic approaches showed that the Nups interact with the LINC complex. These results suggest that Nups regulate centromere scattering cooperatively with condensin II and the LINC complex.

SUBMITTER: Ito N

PROVIDER: S-EPMC10839643 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Nuclear pore complex proteins are involved in centromere distribution.

Ito Nanami N Sakamoto Takuya T Oko Yuka Y Sato Hikaru H Hanamata Shigeru S Sakamoto Yuki Y Matsunaga Sachihiro S

iScience 20240111 2

The subnuclear distribution of centromeres is cooperatively regulated by condensin II and the linker of nucleoskeleton and cytoskeleton (LINC) complex. However, other nuclear membrane structures and nuclear proteins are probably involved in centromere dynamics and distribution. Here, we focused on the nuclear pore complex (NPC), which is known to regulate gene expression, transcription memory, and chromatin structure in addition to transport between the cytoplasm and nucleoplasm. We report here ...[more]

PMID: 38318384

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