Reference Susceptibility Testing and Genomic Surveillance of Clostridioides difficile, United States, 2012-17.

ABSTRACT:

Background

Antimicrobial susceptibility testing (AST) is not routinely performed for Clostridioides difficile and data evaluating minimum inhibitory concentrations (MICs) are limited. We performed AST and whole genome sequencing (WGS) for 593 C. difficile isolates collected between 2012 and 2017 through the Centers for Disease Control and Prevention's Emerging Infections Program.

Methods

MICs to 6 antimicrobial agents (ceftriaxone, clindamycin, meropenem, metronidazole, moxifloxacin, and vancomycin) were determined using the reference agar dilution method according to Clinical and Laboratory Standards Institute guidelines. Whole genome sequencing was performed on all isolates to detect the presence of genes or mutations previously associated with resistance.

Results

A

SUBMITTER: Gargis AS 

PROVIDER: S-EPMC10839785 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature