Determining Collisional Cross Sections from Ion Decay with Individual Ion Mass Spectrometry.

ABSTRACT: Collision cross section (CCS) measurements determined by ion mobility spectrometry (IMS) provide useful information about gas-phase protein structure that is complementary to mass analysis. Methods for determining CCS without a dedicated IMS system have been developed for Fourier transform mass spectrometry (FT-MS) platforms by measuring the signal decay during detection. Individual ion mass spectrometry (I²MS) provides charge detection and measures ion lifetimes across the length of an FT-MS detection event. By tracking lifetimes for entire ion populations, we demonstrate simultaneous determination of charge, mass, and CCS for proteins and complexes ranging from ∼8 to ∼232 kDa.

SUBMITTER: Fisher NP 

PROVIDER: S-EPMC10840072 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature