Advancing Intact Protein Quantitation with Updated Deconvolution Routines.

ABSTRACT: Analysis of intact proteins by mass spectrometry enables direct quantitation of the specific proteoforms present in a sample and is an increasingly important tool for biopharmaceutical and academic research. Interpreting and quantifying intact protein species from mass spectra typically involves many challenges including mass deconvolution and peak processing as well as determining optimal spectral averaging parameters and matching masses to theoretical proteoforms. Each of these steps can present informatic hurdles, as parameters often need to be tailored specifically to the data sets. To reduce intact mass deconvolution data analysis burdens, we built upon the widely used "sliding window" mass deconvolution technique with several additional concepts. First, we found that how spectra are

SUBMITTER: Robey MT 

PROVIDER: S-EPMC10840078 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature