Tunable PhenoCycler imaging of the murine pre-clinical tumour microenvironments.

ABSTRACT:

Background

The tumour microenvironment (TME) consists of tumour-supportive immune cells, endothelial cells, and fibroblasts. PhenoCycler, a high-plex single cell spatial biology imaging platform, is used to characterize the complexity of the TME. Researchers worldwide harvest and bank tissues from mouse models which are employed to model a plethora of human disease. With the explosion of interest in spatial biology, these panoplies of archival tissues provide a valuable resource to answer new questions. Here, we describe our protocols for developing tunable PhenoCycler multiplexed imaging panels and describe our open-source data analysis pipeline. Using these protocols, we used PhenoCycler to spatially resolve the TME of 8 routinely employed pre-clinical models of lymphoma, breast

SUBMITTER: Abraham MJ 

PROVIDER: S-EPMC10840224 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature