Coupled strategy based on regulator manipulation and medium optimization empowers the biosynthetic overproduction of lincomycin.

ABSTRACT: The biosynthesis of bioactive secondary metabolites, specifically antibiotics, is of great scientific and economic importance. The control of antibiotic production typically involves different processes and molecular mechanism. Despite numerous efforts to improve antibiotic yields, joint engineering strategies for combining genetic manipulation with fermentation optimization remain finite. Lincomycin A (Lin-A), a lincosamide antibiotic, is industrially fermented by Streptomyces lincolnensis. Herein, the leucine-responsive regulatory protein (Lrp)-type regulator SLCG_4846 was confirmed to directly inhibit the lincomycin biosynthesis, whereas indirectly controlled the transcription of SLCG_2919, the first reported repressor in S. lincolnensis. Inactivation of SLCG_484

SUBMITTER: Cai X 

PROVIDER: S-EPMC10840354 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature