Isolation and Proteomic Analysis of Extracellular Vesicles from Lactobacillus salivarius SNK-6.

ABSTRACT: The proteins carried by the extracellular vesicles of Lactobacillus salivarius SNK-6 (LsEVs) were identified to provide a foundation for further explorations of the probiotic activities of L. salivarius SNK-6. LsEVs were isolated from the culture media of L. salivarius SNK-6 and morphological analysis was conducted by scanning electron microscopy. Subsequent transmission electron microscopy and nanoparticle tracking analysis were performed to assess the morphology and particle size of the LsEVs. In addition, the protein composition of LsEVs was analyzed using silver staining and protein mass spectrometry. Finally, internalization of the identified LsEVs was confirmed using a confocal microscope, and enzyme-linked immunosorbent assay was employed to analyze the levels o

SUBMITTER: Huang J 

PROVIDER: S-EPMC10840465 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature