Sensitive and Extraction-Free Detection of Methicillin-Resistant Staphylococcus aureus through Ag⁺ Aptamer-Based Color Reaction.

ABSTRACT: Refractory infections, such as hospital-acquired pneumonia, can be better diagnosed with the assistance of precise methicillin-resistant Staphylococcus aureus (MRSA) testing. However, traditional methods necessitate high-tech tools, rigorous temperature cycling, and the extraction of genetic material from MRSA cells. Herein, we propose a sensitive, specific, and extraction-free strategy for MRSA detection by integrating allosteric probe-based target recognition and exonuclease-III (Exo-III)-enhanced color reaction. The penicillin-binding protein 2a (PBP2a) aptamer in the allosteric probe binds with MRSA to convert protein signals to nucleic acid signals. This is followed by the DNA polymerase-assisted target recycle and the production of numerous single-strand DNA (ssDNA) chains whi

SUBMITTER: Cao H 

PROVIDER: S-EPMC10840478 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature