Study Transportation of Drugs within Newly Established Murine Colon Organoid Systems.

ABSTRACT: The development of 3D organoids of the small intestine is a tremendous breakthrough in drug development and biological research. However, the development of colonic organoids (i.e., colonoids) is particularly challenging due to a lack of simple, cost-effective protocols for colonoid cultivation. Here, intestinal homogenates are described as a supplement to the culture medium for maintaining and replicating colonic stem cells. Colonoids generated by this cultivation protocol demonstrate substantial proliferation and differentiation (3 months). There is a similarity between cultured colonoids and primary colon tissue regarding structure and functionality. To evaluate the functionality of colonoids, permeability testing is performed with suspensions of 4 and 40 kDa fluorescein isothiocyanate-

SUBMITTER: Davoudi Z 

PROVIDER: S-EPMC10840714 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature