Ferroptosis of Microglia in Aging Human White Matter Injury.

ABSTRACT:

Objective

Because the role of white matter (WM) degenerating microglia (DM) in remyelination failure is unclear, we sought to define the core features of this novel population of aging human microglia.

Methods

We analyzed postmortem human brain tissue to define a population of DM in aging WM lesions. We used immunofluorescence staining and gene expression analysis to investigate molecular mechanisms related to the degeneration of DM.

Results

We found that DM, which accumulated myelin debris were selectively enriched in the iron-binding protein light chain ferritin, and accumulated PLIN2-labeled lipid droplets. DM displayed lipid peroxidation injury and enhanced expression for TOM20, a mitochondrial translocase, and a sensor of oxidative stress. DM also displayed enhan

SUBMITTER: Adeniyi PA 

PROVIDER: S-EPMC10840747 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature