Monolithic affinity columns in 3D printed microfluidics for chikungunya RNA detection.

ABSTRACT: Mosquito-borne pathogens plague much of the world, yet rapid and simple diagnosis is not available for many affected patients. Using a custom stereolithography 3D printer, we created microfluidic devices with affinity monoliths that could retain, noncovalently attach a fluorescent tag, and detect oligonucleotide and viral RNA. We optimized the fluorescent binding and sample load times using an oligonucleotide sequence from chikungunya virus (CHIKV). We also tested the specificity of CHIKV capture relative to genetically similar Sindbis virus. Moreover, viral RNA from both viruses was flowed through capture columns to study the efficiency and specificity of the column for viral CHIKV. We detected ~10⁷ loaded viral genome copies, which was similar to levels in clinical samples dur

SUBMITTER: Nielsen JB 

PROVIDER: S-EPMC10840819 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature