Intrastromal versus subconjunctival injection of mesenchymal stem/stromal cells for promoting corneal repair.

ABSTRACT:

Purpose

Different approaches to delivery of mesenchymal stem/stromal cells (MSCs) for ameliorating corneal injuries have been investigated. This study was aimed to compare the efficacy of intrastromal and subconjunctival injection of human bone marrow-derived MSCs (hBM-MSCs) in a corneal epithelial injury model.

Methods

Twenty-four C57BL/6J mice underwent total corneal and limbal epithelial debridement. Then, the mice were divided into three different groups: (1) intrastromal hBM-MSCs injection, (2) subconjunctival hBM-MSCs injection, and (3) injection of frozen medium as a control. Mice were monitored by slit lamp and underwent anterior segment optical coherence tomography (ASOCT). Following euthanasia, the corneas were further evaluated by histology and immunostaining.

SUBMITTER: Soleimani M 

PROVIDER: S-EPMC10841412 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature