Label-free imaging of nuclear membrane for analysis of nuclear import of viral complexes.

ABSTRACT: HIV-1 enters the nucleus of non-dividing cells through the nuclear pore complex where it integrates into the host genome. The mechanism of HIV-1 nuclear import remains poorly understood. A powerful means to investigate the docking of HIV-1 at the nuclear pore and nuclear import of viral complexes is through single virus tracking in live cells. This approach necessitates fluorescence labeling of HIV-1 particles and the nuclear envelope, which may be challenging, especially in the context of primary cells. Here, we leveraged a deep neural network model for label-free visualization of the nuclear envelope using transmitted light microscopy. A training image set of cells with fluorescently labeled nuclear Lamin B1 (ground truth), along with the corresponding transmitted light images, was acqui

SUBMITTER: Ten Eyck A 

PROVIDER: S-EPMC10841631 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature