Thrombomodulin Regulates PTEN/AKT Signaling Axis in Endothelial Cells.

ABSTRACT:

Background

We recently demonstrated that deletion of thrombomodulin gene from endothelial cells results in upregulation of proinflammatory phenotype. In this study, we investigated the molecular basis for the altered phenotype in thrombomodulin-deficient (TM^-/-) cells.

Methods

Different constructs containing deletions or mutations in the cytoplasmic domain of thrombomodulin were prepared and introduced to TM^-/- cells. The phenotype of cells expressing different derivatives of thrombomodulin and tissue samples of thrombomodulin-knockout mice were analyzed for expression of distinct regulatory genes in established signaling assays.

Results

The phosphatase and tensin homolog were phosphorylated and its recruitment to the plasma membrane was impaired

SUBMITTER: Giri H 

PROVIDER: S-EPMC10841639 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature