Sex-Linked Discrepancies in C57BL6/J Mouse Osteoarthritis are Associated With the Gut Microbiome and are Transferrable by Microbiome Transplantation.

ABSTRACT:

Objective

Females have reduced osteoarthritis (OA) in surgical models. The objective of the current study was to evaluate a sex-linked gut microbiome in the pathogenesis of OA.

Methods

We induced OA via destabilization of the medial meniscus surgery in adult male and female C57BL6/J mice with and without opposite-sex microbiome transplantation. Eight weeks later, animals were euthanized, and OA severity, synovitis, and osteophyte scores were determined. Serum lipopolysaccharide was measured chromogenically, and serum cytokines were quantified via multiplex immunoassay. Cecal microbiome profiles were generated using 16S deep sequencing.

Results

Males had worse OA histology (3.5x, P = 6 × 10^-7 ), synovitis (2.4x, P = 5 × 10^-4 ), and osteophyte scor

SUBMITTER: Schlupp L 

PROVIDER: S-EPMC10842085 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature