K_ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production.

ABSTRACT: Pancreatic beta cells secrete insulin in response to plasma glucose. The ATP-sensitive potassium channel (K_ATP ) links glucose metabolism to islet electrical activity in these cells by responding to increased cytosolic [ATP]/[ADP]. It was recently proposed that pyruvate kinase (PK) in close proximity to beta cell K_ATP locally produces the ATP that inhibits K_ATP activity. This proposal was largely based on the observation that applying phosphoenolpyruvate (PEP) and ADP to the cytoplasmic side of excised inside-out patches inhibited K_ATP . To test the relative contributions of local vs. mitochondrial ATP production, we recorded K_ATP activity using mouse beta cells and INS-1 832/13 cells. In contrast to prior reports, we could not replica

SUBMITTER: Corradi J 

PROVIDER: S-EPMC10842208 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature