Simultaneous creatine and phosphocreatine mapping of skeletal muscle by CEST MRI at 3T.

ABSTRACT:

Purpose

To confirm that CrCEST in muscle exhibits a slow-exchanging process, and to obtain high-resolution amide, creatine (Cr), and phosphocreatine (PCr) maps of skeletal muscle using a POlynomial and Lorentzian Line-shape Fitting (PLOF) CEST at 3T.

Methods

We used dynamic changes in PCr/CrCEST of mouse hindlimb before and after euthanasia to assign the Cr and PCr CEST peaks in the Z-spectrum at 3T and to obtain the optimum saturation parameters. Segmented 3D EPI was employed to obtain multi-slice amide, PCr, and Cr CEST maps of human skeletal muscle. Subsequently, the PCrCEST maps were calibrated using the PCr concentrations determined by ³¹ P MRS.

Results

A comparison of the Z-spectra in mouse hindlimb before and after euthanasia indicated that CrCEST is

SUBMITTER: Ju L 

PROVIDER: S-EPMC10842434 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature