Effects of storage temperature and time on metabolite profiles measured in dried blood spots, dried blood microsamplers, and plasma.

ABSTRACT: The practical advantages of capillary whole blood collection over venipuncture plasma collection for human exposome research are well known. However, before epidemiologists, clinicians, and public health researchers employ these microvolume sample collections, a rigorous evaluation of pre-analytical storage conditions is needed to develop protocols that maximize sample stability and reliability over time. Therefore, we performed a controlled experiment of dried whole blood collected on 10 μL Mitra microsamplers (DBM), 5-mm punches of whole blood from a dried blood spot (DBS), and 10 μL of plasma, and evaluated the effects of storage conditions at 4 °C, -20 °C, or -80 °C for up to 6 months on the resulting metabolite profiles measured with untargeted liquid chromatography-high resolution ma

SUBMITTER: Petrick LM 

PROVIDER: S-EPMC10842436 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature