A high-throughput single-cell RNA expression profiling method identifies human pericyte markers.

ABSTRACT:

Aims

We sought to identify and optimise a universally available histological marker for pericytes in the human brain. Such a marker could be a useful tool for researchers. Further, identifying a gene expressed relatively specifically in human pericytes could provide new insights into the biological functions of this fascinating cell type.

Methods

We analysed single-cell RNA expression profiles derived from different human and mouse brain regions using a high-throughput and low-cost single-cell transcriptome sequencing method called EasySci. Through this analysis, we were able to identify specific gene markers for pericytes, some of which had not been previously characterised. We then used commercially (and therefore universally) available antibodies to immunolabel the pericy

SUBMITTER: Sziraki A 

PROVIDER: S-EPMC10842535 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature