Detection of High-Risk Paraneoplastic Antibodies against TRIM9 and TRIM67 Proteins.

ABSTRACT:

Objective

Co-occurring anti-tripartite motif-containing protein 9 and 67 autoantibodies (TRIM9/67-IgG) have been reported in only a very few cases of paraneoplastic cerebellar syndrome. The value of these biomarkers and the most sensitive methods of TRIM9/67-IgG detection are not known.

Methods

We performed a retrospective, multicenter study to evaluate the cerebrospinal fluid and serum of candidate TRIM9/67-IgG cases by tissue-based immunofluorescence, peptide phage display immunoprecipitation sequencing, overexpression cell-based assay (CBA), and immunoblot. Cases in which TRIM9/67-IgG was detected by at least 2 assays were considered TRIM9/67-IgG positive.

Results

Among these cases (n = 13), CBA was the most sensitive (100%) and revealed that all cases had TRIM9 an

SUBMITTER: Bartley CM 

PROVIDER: S-EPMC10842626 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature