Examining memory linking and generalization using scFLARE2, a temporally precise neuronal activity tagging system.

ABSTRACT: How memories are organized in the brain influences whether they are remembered discretely versus linked with other experiences or whether generalized information is applied to entirely novel situations. Here, we used scFLARE2 (single-chain fast light- and activity-regulated expression 2), a temporally precise tagging system, to manipulate mouse lateral amygdala neurons active during one of two 3 min threat experiences occurring close (3 h) or further apart (27 h) in time. Silencing scFLARE2-tagged neurons showed that two threat experiences occurring at distal times are dis-allocated to orthogonal engram ensembles and remembered discretely, whereas the same two threat experiences occurring in close temporal proximity are linked via co-allocation to overlapping engram ensembles. Moreover, we

SUBMITTER: Jung JH 

PROVIDER: S-EPMC10842737 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature