Epileptic activity triggers rapid ROCK1-dependent astrocyte morphology changes.

ABSTRACT: Long-term modifications of astrocyte function and morphology are well known to occur in epilepsy. They are implicated in the development and manifestation of the disease, but the relevant mechanisms and their pathophysiological role are not firmly established. For instance, it is unclear how quickly the onset of epileptic activity triggers astrocyte morphology changes and what the relevant molecular signals are. We therefore used two-photon excitation fluorescence microscopy to monitor astrocyte morphology in parallel to the induction of epileptiform activity. We uncovered astrocyte morphology changes within 10-20 min under various experimental conditions in acute hippocampal slices. In vivo, induction of status epilepticus resulted in similarly altered astrocyte morphology within 30 min.

SUBMITTER: Anders S 

PROVIDER: S-EPMC10842783 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature