Using dynamic biomaterials to study the temporal role of bioactive peptides during osteogenesis.

ABSTRACT: The extracellular matrix is a highly dynamic environment, and the precise temporal presentation of biochemical signals is critical for regulating cell behavior during development, healing, and disease progression. To mimic this behavior, we developed a modular DNA-based hydrogel platform to enable independent and reversible control over the immobilization of multiple biomolecules during in vitro cell culture. We combined reversible DNA handles with a norbornene-modified hyaluronic acid hydrogel to orthogonally add and remove multiple biomolecule-DNA conjugates at user-defined timepoints. We demonstrated that the persistent presentation of the cell adhesion peptide RGD was required to maintain cell spreading on hyaluronic acid hydrogels. Further, we discovered the delayed presentation of os

SUBMITTER: Fumasi FM 

PROVIDER: S-EPMC10842892 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature