Dataset Information

Exploring the mechanism of LncRNA CASC15 affecting hepatocellular carcinoma through miRNA.

ABSTRACT: This study aimed to determine the potential mechanisms through which long noncoding (Lnc) RNA cancer susceptibility candidate 15 (CASC15) affects hepatocellular carcinoma (HCC). We retrieved HCC RNA-seq and clinical information from the UCSC Xena database. The differential expression (DE) of CASC15 was detected. Overall survival was analyzed using Kaplan-Meier (K-M) curves. Molecular function and signaling pathways affected by CASC15 were determined using Gene Set Enrichment Analysis. Associations between CASC15 and the HCC microenvironment were investigated using immuno-infiltration assays. A differential CASC15-miRNA-mRNA network and HCC-specific CASC15-miRNA-mRNA ceRNA network were constructed. The overexpression of CASC15 in HCC tissues was associated with histological grade, clinical stage, pathological T stage, poor survival, more complex immune cell components, and 12 immune checkpoints. We identified 27 DE miRNAs and 270 DE mRNAs in the differential CASC15-miRNA-mRNA network, and 10 key genes that were enriched in 12 cancer-related signaling pathways. Extraction of the HCC-specific CASC15-miRNA-mRNA network revealed that IGF1R, MET, and KRAS were associated with HCC progression and occurrence. Our bioinformatic findings confirmed that CASC15 is a promising prognostic biomarker for HCC, and elevated levels in HCC are associated with the tumor microenvironment. We also constructed a disease-specific CASC15-miRNA-mRNA regulatory ceRNA network that provides a new perspective for the precise indexing of patients with elevated levels of CASC15.

SUBMITTER: Cai Q

PROVIDER: S-EPMC10843454 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Exploring the mechanism of LncRNA CASC15 affecting hepatocellular carcinoma through miRNA.

Cai Qingshan Q Wu Dongyang D Shen Yueling Y Li Shudong S Liu Liyou L Liu Dong D Li Yong Y Chen Xiaonan X Wang Limin L Zheng Jianxing J

Medicine 20240201 5

This study aimed to determine the potential mechanisms through which long noncoding (Lnc) RNA cancer susceptibility candidate 15 (CASC15) affects hepatocellular carcinoma (HCC). We retrieved HCC RNA-seq and clinical information from the UCSC Xena database. The differential expression (DE) of CASC15 was detected. Overall survival was analyzed using Kaplan-Meier (K-M) curves. Molecular function and signaling pathways affected by CASC15 were determined using Gene Set Enrichment Analysis. Associatio ...[more]

PMID: 38306545

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Dataset Information

Exploring the mechanism of LncRNA CASC15 affecting hepatocellular carcinoma through miRNA.

Publications

Exploring the mechanism of LncRNA CASC15 affecting hepatocellular carcinoma through miRNA.

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