A protocol for creating endometriosis in rhesus macaques (Macaca mulatta).

ABSTRACT:

Background

Endometriosis is the presence of endometrium-like tissue outside the uterine cavity. An experimental model of endometriosis has been created in the baboon by the transcervical collection and laparoscopic inoculation of menstrual endometrium. Macaques are the preferred model for pharmaceutical development, but the complex anatomy of the macaque cervix makes the baboon method impractical. In this work, we sought to validate a surgical approach for creating endometriosis in macaques.

Methods

Menstrual endometrium was collected via laparoscopic intrauterine puncture and transferred to the peritoneal cavity. We repeated this procedure during three menstruations. Endometriotic tissue was identified during laparoscopy, collected, and characterized by immunohistochemistry

SUBMITTER: Slayden OD 

PROVIDER: S-EPMC10843508 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature