Design of an Ara h 2 hypoallergen from conformational epitopes.

ABSTRACT:

Introduction

Adverse reactions are relatively common during peanut oral immunotherapy. To reduce the risk to the patient, some researchers have proposed modifying the allergen to reduce IgE reactivity, creating a putative hypoallergen. Analysis of recently cloned human IgG from patients treated with peanut immunotherapy suggested that there are three common conformational epitopes for the major peanut allergen Ara h 2. We sought to test if structural information on these epitopes could indicate mutagenesis targets for designing a hypoallergen and evaluated the reduction in IgE binding via immunochemistry and a mouse model of passive cutaneous anaphylaxis (PCA).

Methods

X-ray crystallography characterized the conformational epitopes in detail, followed by mutational analysis

SUBMITTER: Min J 

PROVIDER: S-EPMC10843581 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature