Direct observation of cortactin protecting Arp2/3-actin filament branch junctions from GMF-mediated destabilization.

ABSTRACT: How cells tightly control the formation and turnover of branched actin filament arrays to drive cell motility, endocytosis, and other cellular processes is still not well understood. Here, we investigated the mechanistic relationship between two binding partners of the Arp2/3 complex, glia maturation factor (GMF) and cortactin. Individually, GMF and cortactin have opposite effects on the stability of actin filament branches, but it is unknown how they work in concert with each other to govern branch turnover. Using TIRF microscopy, we observe that GMF's branch destabilizing activities are potently blocked by cortactin (IC₅₀ = 1.3 nM) and that this inhibition requires direct interactions of cortactin with Arp2/3 complex. The simplest model that would explain these results is comp

SUBMITTER: McGuirk ER 

PROVIDER: S-EPMC10843626 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature