Phenotypic signatures of circulating neoantigen-reactive CD8⁺ T cells in patients with metastatic cancers.

ABSTRACT: Circulating T cells from peripheral blood (PBL) can provide a rich and noninvasive source for antitumor T cells. By single-cell transcriptomic profiling of 36 neoantigen-specific T cell clones from 6 metastatic cancer patients, we report the transcriptional and cell surface signatures of antitumor PBL-derived CD8⁺ T cells (NeoTCR_PBL). Comparison of tumor-infiltrating lymphocyte (TIL)- and PBL-neoantigen-specific T cells revealed that NeoTCR_PBL T cells are low in frequency and display less-dysfunctional memory phenotypes relative to their TIL counterparts. Analysis of 100 antitumor TCR clonotypes indicates that most NeoTCR_PBL populations target the same neoantigens as TILs. However, NeoTCR_PBL TCR repertoire is only partially shared with

SUBMITTER: Yossef R 

PROVIDER: S-EPMC10843665 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature