Improving the efficiency of DNA extraction from iron incrustations and oilfield-produced water.

ABSTRACT: The quantity and quality of DNA isolated from environmental samples are crucial for getting robust high-throughput sequencing data commonly used for microbial community analysis. The differences in the nature and physicochemical properties of environmental samples impact DNA yields, and therefore, an optimisation of the protocols is always recommended. For instance, samples collected from corroded areas contain high concentrations of metals, salts, and hydrocarbons that can interfere with several steps of the DNA extraction protocols, thereby reducing yield and quality. In this study, we compared the efficiency of commercially available DNA extraction kits and laboratory-adopted methods for microbial community analysis of iron incrustations and oilfield-produced water samples. Modification

SUBMITTER: Foysal MJ 

PROVIDER: S-EPMC10844625 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature