Urine-derived bladder cancer organoids (urinoids) as a tool for cancer longitudinal response monitoring and therapy adaptation.

ABSTRACT:

Background

Bladder cancer is one of the most common cancer types worldwide. Generally, research relies on invasive sampling strategies.

Methods

Here, we generate bladder cancer organoids directly from urine (urinoids). In this project, we establish 12 urinoid lines from 22 patients with non-muscle and muscle-invasive bladder tumours, with an efficiency of 55%.

Results

The histopathological features of the urinoids accurately resemble those of the original bladder tumours. Genetically, there is a high concordance of single nucleotide polymorphisms (92.56%) and insertions & deletions (91.54%) between urinoids and original tumours from patient 4. Furthermore, these urinoids show sensitivity to bladder cancer drugs, similar to their tissue-derived organoid counterparts. G

SUBMITTER: Viergever BJ 

PROVIDER: S-EPMC10844626 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature