Nur77 mitigates endothelial dysfunction through activation of both nitric oxide production and anti-oxidant pathways.

ABSTRACT:

Background

Nur77 belongs to the member of orphan nuclear receptor 4A family that plays critical roles in maintaining vascular homeostasis. This study aims to determine whether Nur77 plays a role in attenuating vascular dysfunction, and if so, to determine the molecular mechanisms involved.

Methods

Both Nur77 knockout (Nur77 KO) and Nur77 endothelial specific transgenic mice (Nur77-Tg) were employed to examine the functional significance of Nur77 in vascular endothelium in vivo. Endothelium-dependent vasodilatation to acetylcholine (Ach) and reactive oxygen species (ROS) production was determined under inflammatory and high glucose conditions. Expression of genes was determined by real-time PCR and western blot analysis.

Results

In response to tumor necrosis factor alp

SUBMITTER: Lu L 

PROVIDER: S-EPMC10844745 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature