Digestive interaction between dietary nitrite and dairy products generates novel nitrated linolenic acid products.

ABSTRACT: Nitrated fatty acids are important anti-inflammatory and protective lipids formed in the gastric compartment, with conjugated linoleic acid (rumenic acid, RA, 9Z,11E-18:2) being the primary substrate for lipid nitration. The recently reported identification of nitrated rumelenic acid (NO₂-RLA) in human urine has led to hypothesize that rumelenic acid (RLA, 9Z,11E,15Z-18:3) from dairy fat is responsible for the formation of NO₂-RLA. To evaluate the source and mechanism of NO₂-RLA formation, ¹⁵N labeled standards of NO₂-RLA were synthesized and characterized. Afterward, milk fat with different RA and RLA levels was administered to mice in the presence of nitrite, and the appearance of nitrated fatty acids in plasma and urine followed. We

SUBMITTER: Salvatore SR 

PROVIDER: S-EPMC10844836 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature