Association between spatial distribution of leukocyte subsets and clinical presentation of head and neck squamous cell carcinoma.

ABSTRACT:

Background

Interactions between tumor cells and cells in the microenvironment contribute to tumor development and metastasis. The spatial arrangement of individual cells in relation to each other influences the likelihood of whether and how these cells interact with each other.

Methods

This study investigated the effect of spatial distribution on the function of leukocyte subsets in the microenvironment of human head and neck squamous cell carcinoma (HNSCC) using multiplex immunohistochemistry (IHC). Leukocyte subsets were further classified based on analysis of two previously published HNSCC single-cell RNA datasets and flow cytometry (FC).

Results

IHC revealed distinct distribution patterns of leukocytes differentiated by CD68 and CD163. While CD68hiCD163^lo

SUBMITTER: Netzer C 

PROVIDER: S-EPMC10844964 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature