An omicron-specific neutralizing antibody test predicts neutralizing activity against XBB 1.5.

ABSTRACT:

Introduction

Understanding the immune status of an individual using neutralizing antibody testing is complicated by the continued evolution of the SARS-CoV-2 virus. Previous work showed that assays developed against the wildtype strain of SARS-CoV-2 were insufficient predictors of neutralization of omicron variants, thus we developed an omicron-specific flow cytometry-based neutralizing antibody test and performed experiments to assess how well it compared to an omicron-specific PRNT assay (gold standard) and whether it could predict neutralizing activity to more recent omicron subvariants such as XBB.1.5.

Methods

Accuracy of a novel flow cytometry-based neutralizing antibody (FC-NAb) assay was determined by comparison with an omicron-specific PRNT assay. A series of samples

SUBMITTER: Varvel S 

PROVIDER: S-EPMC10845052 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature