Cost-effective strategies to knock down genes of interest in the retinas of adult zebrafish.

ABSTRACT: High throughput sequencing has generated an enormous amount of information about the genes expressed in various cell types and tissues throughout the body, and about how gene expression changes over time and in diseased conditions. This knowledge has made targeted gene knockdowns an important tool in screening and identifying the roles of genes that are differentially expressed among specific cells of interest. While many approaches are available and optimized in mammalian models, there are still several limitations in the zebrafish model. In this article, we describe two approaches to target specific genes in the retina for knockdown: cell-penetrating, translation-blocking Vivo-Morpholino oligonucleotides and commercially available lipid nanoparticle reagents to deliver siRNA. We targeted

SUBMITTER: Shihabeddin E 

PROVIDER: S-EPMC10845135 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature