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Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts.

ABSTRACT: Curative bone marrow transplantation (BMT) therapies for sickle cell disease (SCD) can cause infertility. The Fertility Preservation Program (FPP) in Pittsburgh cryopreserves testicular tissues for SCD patients prior to BMT in anticipation that those tissues can be thawed in the future and matured to produce sperm. Here, we generated and validated two isogenic patient-derived induced pluripotent stem cell (iPSC) lines from testicular biopsy fibroblasts of a 12-year-old SCD patient.

SUBMITTER: Harancher MR

PROVIDER: S-EPMC10845156 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts.

Harancher Mitchell R MR Sukhwani Meena M Castro Carlos A CA Hsieh Michael H MH Orwig Kyle E KE

Stem cell research 20231121

Curative bone marrow transplantation (BMT) therapies for sickle cell disease (SCD) can cause infertility. The Fertility Preservation Program (FPP) in Pittsburgh cryopreserves testicular tissues for SCD patients prior to BMT in anticipation that those tissues can be thawed in the future and matured to produce sperm. Here, we generated and validated two isogenic patient-derived induced pluripotent stem cell (iPSC) lines from testicular biopsy fibroblasts of a 12-year-old SCD patient. ...[more]

PMID: 38000347

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