Recovering high-quality bacterial genomes from cross-contaminated cultures: a case study of marine Vibrio campbellii.

ABSTRACT:

Background

Environmental monitoring of bacterial pathogens is critical for disease control in coastal marine ecosystems to maintain animal welfare and ecosystem function and to prevent significant economic losses. This requires accurate taxonomic identification of environmental bacterial pathogens, which often cannot be achieved by commonly used genetic markers (e.g., 16S rRNA gene), and an understanding of their pathogenic potential based on the information encoded in their genomes. The decreasing costs of whole genome sequencing (WGS), combined with newly developed bioinformatics tools, now make it possible to unravel the full potential of environmental pathogens, beyond traditional microbiological approaches. However, obtaining a high-quality bacterial genome, requires initial c

SUBMITTER: Orel N 

PROVIDER: S-EPMC10845552 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature