HAND factors regulate cardiac lineage commitment and differentiation from human pluripotent stem cells.

ABSTRACT:

Background

Transcription factors HAND1 and HAND2 (HAND1/2) play significant roles in cardiac organogenesis. Abnormal expression and deficiency of HAND1/2 result in severe cardiac defects. However, the function and mechanism of HAND1/2 in regulating human early cardiac lineage commitment and differentiation are still unclear.

Methods

With NKX2.5^eGFP H9 human embryonic stem cells (hESCs), we established single and double knockout cell lines for HAND1 and HAND2, respectively, whose cardiomyocyte differentiation efficiency could be monitored by assessing NKX2.5-eGFP⁺ cells with flow cytometry. The expression of specific markers for heart fields and cardiomyocyte subtypes was examined by quantitative PCR, western blot and immunofluorescence staining. Microel

SUBMITTER: Guo H 

PROVIDER: S-EPMC10845658 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature