Rapid detection of Staphylococcus aureus in blood culture samples using human IgG-based lateral flow assay.

ABSTRACT: Staphylococcus aureus is one of the most common pathogens. The conventional workflow for identifying this organism is time-consuming and takes up to several days. Therefore, we developed a colloidal gold-based lateral flow immunoassay (LFIA) using human IgG as a conjugated antibody to detect S. aureus. One hundred and thirty-eight clinical isolates, including 79 S. aureus and 59 non-S. aureus were spiked in blood samples, and incubated at 37°C for 24 h. The bacterial antigens were simply extracted before being tested by the developed LFIA strips. The results were read by the naked eye within 15 min. Conventional PCR was used as a reference method. The sensitivity and specificity of the developed LFIA were 100% (95% CI: 94.2%-100.0% and 92.4%-100.0%

SUBMITTER: Srisrattakarn A 

PROVIDER: S-EPMC10846088 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature