fosA11, a novel chromosomal-encoded fosfomycin resistance gene identified in Providencia rettgeri.

ABSTRACT: This study investigated resistance genes corresponding to the fosfomycin resistance phenotype in clinical isolate Providencia rettgeri W986, as well as characterizing the enzymatic activity of FosA11 and the genetic environment. Antimicrobial susceptibility testing was performed using the agar microdilution method based on the Clinical and Laboratory Standards Institute guidelines. The whole genomic sequence of Providencia rettgeri W986 was obtained using Illumina sequencing and the PacBio platform. The fosA-11 gene was amplified by PCR and cloned into the pUCP20 vector. The recombinant strain pCold1-fosA11-BL21 was expressed to extract the target protein, and absorbance photometry was applied for enzymatic parameter determination. Minimal inhibitory concentrati

SUBMITTER: Lu W 

PROVIDER: S-EPMC10846113 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature