Unveiling the super tolerance of Candida nivariensis to oxidative stress: insights into the involvement of a catalase.

ABSTRACT: Yeast cells involved in fermentation processes face various stressors that disrupt redox homeostasis and cause cellular damage, making the study of oxidative stress mechanisms crucial. In this investigation, we isolated a resilient yeast strain, Candida nivariensis GXAS-CN, capable of thriving in the presence of high concentrations of H₂O₂. Transcriptomic analysis revealed the up-regulation of multiple antioxidant genes in response to oxidative stress. Deletion of the catalase gene Cncat significantly impacted H₂O₂-induced oxidative stress. Enzymatic analysis of recombinant CnCat highlighted its highly efficient catalase activity and its essential role in mitigating H₂O₂. Furthermore, over-expression of

SUBMITTER: Qi Y 

PROVIDER: S-EPMC10846165 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature